Table 10.(Continued) %o Triphosphate Form Months Powder dgTP 9883 95.47 0.5 dTTP 98.87 duTP 98.02 71.55 90.1 40.13(6mo) Solution(10mM) dATP 9968 dcTP 982 9885(2mo 98.6 9951 dTTP 93.57 9929 dUTP 93.8 9945 (12mo) 98.5(2mo Solution ddNTP(10mM) ddATP 99.69 94.52 ddCTP 984 94. 9936 Solution ddNTP(5mM) ddATP 68.56 9963 ddcTP 100 ddGTP 34343 96.67 ddTTP RTP Solutions(100mM) ATP 9857 98.18 95.39 CTP 333 99 98.43 GTP 9846 98.44 96.82 UTP 9971 Source: Data aration of nucleotide species via high perfor on an Amersham Pharmacia Biotech FPLCe Syster Notes: Each umoles(0. 2 ml of a 1 mM solution) was injected onto a Mono QB Ion Exchange column Using the following buffers: Buffer A, 5mM sodium phosphate, PH 7.0 Buffer B, 5mM sodium phosphate, IM NaCl PH 7.0. trification was achieved via a gradient of 5-35% NaCl over 15 minutes using a flow rate Nucleotides, Oligonucleotides, and Polynucleotides 271Nucleotides, Oligonucleotides, and Polynucleotides 271 Table 10.1 (Continued) % Triphosphate Form Months -70°C -20°C 4°C 21°C Powder dGTP 54 99.63 98.83 95.47 90.5 (2 mo) 19.7 (42 mo) dTTP 54 99.44 98.87 93.54 95.6 (2 mo) 0.07 (42) dUTP 54 99.23 98.02 71.55 90.1 (1.2mo) 40.13 (6mo) Solution (10 mM) dATP 15 99.68 99.59 88.6 (12 mo) 98.5 (2 mo) dCTP 15 98.2 99.56 86.11 (12 mo) 98.85 (2mo) dGTP 15 98.6 99.51 89.47 (12 mo) 98.35 (2mo) dTTP 15 93.57 99.29 81.05 (12 mo) 98.86 (2mo) dUTP 15 93.8 99.45 84.95 (12 mo) 98.5 (2mo) Solution ddNTP (10 mM) ddATP 3 99.69 99.49 94.52 ddCTP 3 100 98.51 97.38 ddGTP 3 98.4 98.08 94.23 ddTTP 3 99.36 99.13 87.06 Solution ddNTP (5 mM) ddATP 3 99.77 98.12 68.56 4 99.63 96.31 2 ddCTP 3 98.77 100 98.4 4 99.27 99.46 93.72 ddGTP 3 95.61 98 96.67 4 98.25 97.9 93.68 ddTTP 3 93.1 55.09 49.03 4 94.25 63.23 3.6 RTP Solutions (100 mM) ATP 3 98.57 98.18 95.39 CTP 3 99.25 99.43 98.43 GTP 3 98.46 98.44 96.82 UTP 3 99.71 99.69 97.99 Source: Data based on chromatographic separation of nucleotide species via high perfor￾mance chromatography on an Amersham Pharmacia Biotech FPLC® System. Notes: Each sample, 0.2mmoles (0.2 ml of a 1 mM solution) was injected onto a Mono Q® Ion Exchange column. Using the following buffers: Buffer A, 5mM sodium phosphate, pH 7.0. Buffer B, 5mM sodium phosphate, 1M NaCl, pH 7.0. purification was achieved via a gradient of 5–35% NaCl over 15 minutes using a flow rate of 1 ml/min. Nucleotide peaks were detected at of 254 nm. (Data from Amersham Phar￾macia Biotech, 1993a.)