Key Steps in Neurotransmission: Synthesis Storage Metabolism Action potential Recognition (action) Strategies for Pharmacological Intervention: Block synthesis and storage: Usually rate-limiting steps;produce long-term effects Block release: Rapid action and effective Interfere with metabolism: Can be reversible or irreversible:blocking metabolism increases effective neurotransmitter concentrations Interfere with action: Receptor antagonists agonists;high specificity Synthesis of acetylcholine: Choline Acetylcholine CH、 CH3 0 CH3-N'-CHz-CHz-OH Choline CH3-N+-CHz-CHz-O-C-CH3 CH3 acetyltransferase CH3+ 0 CoA-SH CoA-S-C-CH3 Acetyl-CoA CoA 55 Synthesis & Storage Action potential Metabolism Recognition (action) Key Steps in Neurotransmission: Strategies for Pharmacological Intervention: Block synthesis and storage: Usually rate-limiting steps; produce long-term effects Block release: Rapid action and effective Interfere with metabolism: Can be reversible or irreversible; blocking metabolism increases effective neurotransmitter concentrations Interfere with action: Receptor antagonists & agonists; high specificity Release Synthesis of acetylcholine: CH3 CH3 CH3 N+–CH2–CH2–OH CoA–S–C–CH3 O Choline Acetyl-CoA + Choline acetyltransferase CH3 CH3 CH3 N+–CH2–CH2–O–C–CH3 O CoA-SH + CoA Acetylcholine