2018/10/27 Saturday How are mass spectra produced Mass Spectrometer Principles Sample lonizer Mass Analyzer Detector Data system 25 How does a mass spectrometer work? Create ions Separate ions Detections ·Ionization ·ass analyzer method -MALDI-TOF ·Mass spectrum MW Database -MALDI -Triple Quadrapole analysis -Electrospray meaidan 258 8 2018/10/27 Saturday 8 255 Mass Spectrometer Principles Ionizer Sample + _ Mass Analyzer Detector Data system How are mass spectra produced ? 256 How does a mass spectrometer work? • Ionization method – MALDI – Electrospray (Proteins must be charged and dry) • Mass analyzer – MALDI-TOF • MW – Triple Quadrapole • AA seq – MALDI-QqTOF • AA seq and MW – QqTOF • AA seq and protein modif. Create ions Separate ions Detect ions • Mass spectrum • Database analysis