9/18/2016 ·Select agents must be Biosafety Levels -inventoried Biosafety levels range from BSL-1 to BSL-4 -kept locked in a room assessable only microbes do not consimimol poreease rd tn me m。po in healtl to people cleared by the Dept of Justice BSL-2-microbes pose moderate hazards to lab -handled using BSL-3 level work workers and the environment and are associated practices High Riak Mlernes with diseases of varying severity BSL-3-microbes cause serious or potentially lethal disease through respiratory transmission BSL-4-microbes are dangerous and exotic,posing 85L2 BSL-1 freatment or vaccines. Disease Characteristics Mild to serious disease that can kill within a day Symptoms 12-36 h but up to 14 days following ingestion of the neurotoxin Earlier the symptoms the more severe the disease First-nausea and vomiting -Then-neurological symptoms >double vision,drooping eyelids >loss of normal mouth and throat function >general fatigue >lack of muscle coordination >respiratory impairment-main cause of death 9/18/2016 4 • Select agents must be - inventoried - kept locked in a room assessable only to people cleared by the Dept of Justice - handled using BSL-3 level work practices Biosafety Levels Biosafety levels range from BSL-1 to BSL-4 • BSL-1 - microbes do not consistently cause disease in healthy adults and present minimal potential hazard to lab workers and the environment • BSL-2 – microbes pose moderate hazards to lab workers and the environment and are associated with diseases of varying severity • BSL-3 – microbes cause serious or potentially lethal disease through respiratory transmission • BSL-4 – microbes are dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections are frequently fatal and without treatment or vaccines. Disease Characteristics • Mild to serious disease that can kill within a day • Symptoms - 12-36 h but up to 14 days following ingestion of the neurotoxin - Earlier the symptoms the more severe the disease - First – nausea and vomiting - Then – neurological symptoms  double vision, droopgy in e elids  loss of normal mouth and throat function  general fatigue  lack of muscle coordination  respiratory impairment – main cause of death