Microbiology of otitis media Bacteria can be isolated from one third of patient who have OME The most common are s pneumonia, H influenzae and m catarrhalis (using traditional culture method) By using PCr these three bacteria were detected in approximately 70% of Chronic middle ear effusionMicrobiology of otitis media ◼ Bacteria can be isolated from one third of patient who have OME. The most common are S pneumonia, H influenzae, and M catarrhalis (using traditional culture method). ◼ By using PCR, these three bacteria were detected in approximately 70% of Chronic middle ear effusion