Quantitative Luminescence Spectrophotometry: Fluorescence Absorbed F =K'(10-I) =K'.2.303bcIo =K.c Only works at low A(<0.05) (i)self quenching(collisions between excited states (ii)self absorption (when absorption and fluorescence band overlap) Lamp Sample aperture Shutter disk A Primary Reference filter photomultiplie condary Sample te hotomultiplie Mirror Sample Reference aperture disk Fig.15-6 CEM 333 page 5.10Quantitative Luminescence Spectrophotometry: F Fluorescence } = K' I( 0 - I) Absorbed 6 7 8 = K'×2.303ebc ×I 0 = K× c Only works at low A (<0.05) (i) self quenching (collisions between excited states (ii) self absorption (when absorption and fluorescence band overlap) Fig. 15-6 CEM 333 page 5.10