Question 2 Consider the following two biologically relevant reactions ADP+Pe ATP △G°=+75kcal/mol Creatine+P<> Creatine Phosphate △G°=+95kcal/mol Creatine is used as a storage form of high-energy phosphate in both the muscles and the brain The enzyme, creatine phosphokinase, transfers the phosphate of aTp to creatine generating creatine phosphate. The reaction is reversible such that when energy demand is high(. g during muscle exertion) creatine phosphate donates its phosphate to ADP to yield ATP a)Write the equation for the reaction in which creatine phosphate is synthesized. Be sure to include the AG for the overall reaction. Would you expect the reaction to be spontaneous as written? Creatine +ATP e ADP+ Creatine Phospate AG=+2.0 kcal/mol As written above, the Ag is positive and thus the reaction would be non-spontaneous b)Draw and label a free energy diagram for the overall reaction as written in(a). Be certain to include the relative energy levels of the substrates and the product, the activation energy and the△G° for the overall reaction reactants y Reaction Progress 7012Fall20037.012 Fall 2003 Question 2 Consider the following two biologically relevant reactions: ADP + Pi ↔ ATP ΔG° = +7.5 kcal/mol Creatine + Pi ↔ Creatine Phosphate ΔG° = +9.5 kcal/mol Creatine is used as a storage form of high-energy phosphate in both the muscles and the brain. The enzyme, creatine phosphokinase, transfers the phosphate of ATP to creatine generating creatine phosphate. The reaction is reversible such that when energy demand is high (e.g. during muscle exertion) creatine phosphate donates its phosphate to ADP to yield ATP. a) Write the equation for the reaction in which creatine phosphate is synthesized. Be sure to include the ΔG° for the overall reaction. Would you expect the reaction to be spontaneous as written? Creatine + ATP ↔ ADP + Creatine Phospate ΔG° = +2.0 kcal/mol As written above, the ΔG° is positive and thus the reaction would be non-spontaneous. b) Draw and label a free energy diagram for the overall reaction as written in (a). Be certain to include the relative energy levels of the substrates and the product, the activation energy and the ΔG° for the overall reaction. free energy Reaction Progress products reactants Ea ∆G