An endonuclease in the cell Now,the target site is left naturally excises the old segment with one edited strand and of DNA and seals the new letters one unedited strand. into the genome. Original Cellular sequence endonuclease gets excised New DNA 今MISMATCH!长 To resolve the mismatch,favoring This nick prompts the cell the permanent installation of the to remake that nicked strand. edited DNA.a different guide RNA using the edited strand as a directs the prime editor to nick the template,thus completing unedited strand. the edit. Editor nicks DNA Fully edited DNA Guide RNA Cas9 RT Brosd institute of MIT and Harvard 2019