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Identifying transformants foreign DNA insert Ill. Distinguishing cells carrying insert- Recombinant containing recombinant molecules from Disrupted plasmid cells carrying vectors without inserts lacz gene AmpR Intact vector. Vector EcoRI no insert with insert sites origin of Disrupted licatio lacz ge lacz gene lacz gene split by Transformation: foreign dNA enters the host cell lacz gen foreign DNA insert intact no product lacz gene transcript lacz X-Gal Blue Selecting cells that have received a plasmid pigment
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