ISSN1000-3061CN11-1998Q生物工程学报 Chin J Biotech l12(11):3553-3557 DNA methylation levels and patterns in dwarf [20 Lang ZB, Wang YH, Tang K, et al. Critical roles of ornamental Cunninghamia lanceolata. Acta Hortic dna demethylation the activation of Sin,2015,42(10):2015-2022( in Chinese) hibino 刘琼瑶,黄华宏,冯惠平,等矮生观赏杉木 ripening-repressed genes in tomato fruit. Proc Natl DNA甲基化的水平与模式分析.园艺学报,2015, Acad Sci USa,2017,1l4(22):E451l-E4519 42(10):2015-2022 [21 Cui X, Lu FL, Qiu Q, et al. REF6 recognizes a [30 Wang ZC, Nie LJ, He YX. The effect of specific DNA sequence to demethylate H3K27me3 5-azacytidine to the DNA methylat morphogenesis character of chrysanthemum during Arabidopsis. Nat Genet, 2019, 48(6): 694-699 in vitro growth. Acta Hoticult Sin, 2009, 36(12) [22] Qiu Q, Mei HL, Deng X, et al. DNA methylation 1783-1790(in Chinese) epels targeting of Arabidopsis REF6. Nat Commun, 王子成,聂丽娟,何艳霞.离体条件下5-氮杂胞 2019,10:2063 嘧啶核苷对菊花DNA甲基化和表型性状的影响 [23 Li Q, Gent JI, Zynda G, et al. RNA-directed dNA 园艺学报,2009,36(12):1783-1790 methylation enforces boundaries between [31 Guo JY. Study on microspore abortion of heterochromatin and euchromatin in the maize cytoplasmatic male sterility line Yamian A[DI genome. Proc Natl Acad Sci USA, 2015, 112(47 Jinzhong: Shanxi Agricultural University, 2013(in 14728-14733 nese [24 Zakrzewski F, Schmidt M, Van Lijsebettens M, et al 郭俊尧.细胞质雄性不育系亚棉A小孢子败育过 DNA methylation of retrotransposons, DNA 程的研究[D].晋中:山西农业大学,2013 transposons and genes in sugar beet(Beta vulgaris [32 Ba QS, Zhang GS, Wang JS, et al. Gene expression L.). Plant J,2017,90(6:1156-1175 and DNA methylation alterations in chemically [25 Wang L, Shi Y, Chang X, et al. DNA methylome induced male sterility anthers in wheat(Triticum analysis provides evidence that the expansion of the aestivum L ) Acta Physiol Plant, 2014, 36(2) tea genome is linked to tE bursts. Plant Biotechnol 503-512 J,2018,17(4):826-835 33 Walker J, Gao HB, Zhang JY, et al [26 Wang HH, Wu SJ, Li FF, et al. Transgene silencing Sexual-lineage-specific DNA methylation regulates caused by 35s promoter methylation in upland meiosis in Arabidopsis. Nat Genet, 2018, 50(1): cotton( Gossypium hirsutum). Cott Sci, 2008, 20(4) 130-137 274-280 [34 Teyssier E, Bernacchia G, Maury S, et al. Tissue [27 Li PC, Bi ZZ, Liang WJ, et al. DNA methylation dependent variations of DNA methylation and involved in regulating drought stress response of endoreduplication levels during tomato fruit potato. Acta Agronom Sin, 2019, 45(10): 1595-1603 development and ripening. Planta, 2008, 228(3) 391-399 李鹏程,毕真真,梁文君,等.DNA甲基化参与调[35] Manning K,ToRM, Poole m,etal. A naturally 控马铃薯干旱胁迫响应.作物学报,2019,45(10) occurring epigenetic mutation in a gene encoding an 1595-1603. SBP-box transcription factor inhibits tomato frui [28 Li XN, Cai YL. Analysis of DNA methylation levels ripening. Nat Genet, 2006, 38(8):948-952 and patterns in dwarf rootstock of prunus mahaleb. [36] Wang L, Xie JH, Hu JT, et al. Comparative Acta Bot Boreali-Occid Sin, 2017, 37(5): 864-871 epigenomics reveals evolution of duplicated genes (in Chinese) in potato and tomato. Plant J, 2017, 93(3): 460-471 李向男,蔡宇良。马哈利樱桃矮化砧的DNA甲基[37] Pan YJ,FuBY, Wang d,etal. Spatial and temporal 化水平及模式分析.西北植物学报,2017,37(5) profiling of DNA methylation induced by drought 864-871 Sci Agricult Sin, 2009, 42(9) Liu QY, Huang HH, Feng HP, et al. Analysis of 3009-3018(in Chinese) http://jourmals.im.ac.cn/cjbenISSN 1000-3061 CN 11-1998/Q 生物工程学报 Chin J Biotech http://journals.im.ac.cn/cjbcn 846 112(11): 3553–3557. [20] Lang ZB, Wang YH, Tang K, et al. Critical roles of DNA demethylation in the activation of ripening-induced genes and inhibition of ripening-repressed genes in tomato fruit. Proc Natl Acad Sci USA, 2017, 114(22): E4511–E4519. [21] Cui X, Lu FL, Qiu Q, et al. REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis. Nat Genet, 2019, 48(6): 694–699. [22] Qiu Q, Mei HL, Deng X, et al. DNA methylation repels targeting of Arabidopsis REF6. Nat Commun, 2019, 10: 2063. [23] Li Q, Gent JI, Zynda G, et al. RNA-directed DNA methylation enforces boundaries between heterochromatin and euchromatin in the maize genome. Proc Natl Acad Sci USA, 2015, 112(47): 14728–14733. [24] Zakrzewski F, Schmidt M, Van Lijsebettens M, et al. DNA methylation of retrotransposons, DNA transposons and genes in sugar beet (Beta vulgaris L.). Plant J, 2017, 90(6): 1156–1175. [25] Wang L, Shi Y, Chang XJ, et al. DNA methylome analysis provides evidence that the expansion of the tea genome is linked to TE bursts. Plant Biotechnol J, 2018, 17(4): 826–835. [26] Wang HH, Wu SJ, Li FF, et al. Transgene silencing caused by 35S promoter methylation in upland cotton (Gossypium hirsutum). Cott Sci, 2008, 20(4): 274–280. [27] Li PC, Bi ZZ, Liang WJ, et al. DNA methylation involved in regulating drought stress response of potato. Acta Agronom Sin, 2019, 45(10): 1595–1603 (in Chinese). 李鹏程, 毕真真, 梁文君, 等. DNA 甲基化参与调 控马铃薯干旱胁迫响应. 作物学报, 2019, 45(10): 1595–1603. [28] Li XN, Cai YL. Analysis of DNA methylation levels and patterns in dwarf rootstock of prunus mahaleb. Acta Bot Boreali-Occid Sin, 2017, 37(5): 864–871 (in Chinese). 李向男, 蔡宇良. 马哈利樱桃矮化砧的 DNA 甲基 化水平及模式分析. 西北植物学报, 2017, 37(5): 864–871. [29] Liu QY, Huang HH, Feng HP, et al. Analysis of DNA methylation levels and patterns in dwarf ornamental Cunninghamia lanceolata. Acta Hortic Sin, 2015, 42(10): 2015–2022 (in Chinese). 刘琼瑶, 黄华宏, 冯惠平, 等. 矮生观赏杉木 DNA 甲基化的水平与模式分析. 园艺学报, 2015, 42(10): 2015–2022. [30] Wang ZC, Nie LJ, He YX. The effect of 5-azacytidine to the DNA methylation and morphogenesis character of chrysanthemum during in vitro growth. Acta Hoticult Sin, 2009, 36(12): 1783–1790 (in Chinese). 王子成, 聂丽娟, 何艳霞. 离体条件下 5-氮杂胞 嘧啶核苷对菊花 DNA 甲基化和表型性状的影响. 园艺学报, 2009, 36(12): 1783–1790. [31] Guo JY. Study on microspore abortion of cytoplasmatic male sterility line Yamian A[D]. Jinzhong: Shanxi Agricultural University, 2013 (in Chinese). 郭俊尧. 细胞质雄性不育系亚棉 A 小孢子败育过 程的研究[D]. 晋中: 山西农业大学, 2013. [32] Ba QS, Zhang GS, Wang JS, et al. Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.). Acta Physiol Plant, 2014, 36(2): 503–512. [33] Walker J, Gao HB, Zhang JY, et al. Sexual-lineage-specific DNA methylation regulates meiosis in Arabidopsis. Nat Genet, 2018, 50(1): 130–137. [34] Teyssier E, Bernacchia G, Maury S, et al. Tissue dependent variations of DNA methylation and endoreduplication levels during tomato fruit development and ripening. Planta, 2008, 228(3): 391–399. [35] Manning K, ToR M, Poole M, et al. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nat Genet, 2006, 38(8): 948–952. [36] Wang L, Xie JH, Hu JT, et al. Comparative epigenomics reveals evolution of duplicated genes in potato and tomato. Plant J, 2017, 93(3): 460–471. [37] Pan YJ, Fu BY, Wang D, et al. Spatial and temporal profiling of DNA methylation induced by drought stress in rice. Sci Agricult Sin, 2009, 42(9): 3009–3018 (in Chinese)