BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 More elegant approaches are now being considered o Colloidal crystal templating Poly(methyl methacrylate) Optical micrograph/20 um pores Fluorescence micrograph/60 um pores 83三 Hydrogel o Nanofiber-based structures(P. Ma) Researchers have also investigated natural materials as scaffolds for tissue engineering- using processed ECM for tissue engineering(we wont pursue here, covered in Biomaterials-Tissue Interactions) o Example materials Decellularized tissues( Badylak 1998, Hilbert 1989) Collagen-based gels(Ellis et al 1996) o Advantages Native cues present Can preserve natural tissue microstructure o Disadvantages: Poor mechanical properties in some cases Difficult to process Poor reproducibility High cost ellular Interactions with Synthetic Degradable Solids Used as Scaffolds? Review older literature looking directly at cells on PLGA, PLA, etc Molecularly-Designed Surfaces for TE Reconstruction at scaffold surfaces Lecture 6- Biodegradable Polymers in Tissue Engineering 3 of 5BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 • More elegant approaches are now being considered: o Poly(methyl methacrylate) microspheres Colloidal crystal templating Hydrogel precursor polymerize Dissolve microspheres Ordered porous structure Optical micrograph/20 µm pores Fluorescence micrograph/60 µm pores 60 µm o Nanofiber-based structures (P. Ma) o • Researchers have also investigated natural materials as scaffolds for tissue engineering- using processed ECM for tissue engineering (we won’t pursue here, covered in Biomaterials-Tissue Interactions) o Example materials ƒ Decellularized tissues (Badylak 1998, Hilbert 1989) ƒ Collagen-based gels (Ellis et al 1996) o Advantages: ƒ Native cues present ƒ Can preserve natural tissue microstructure o Disadvantages: ƒ Poor mechanical properties in some cases ƒ Difficult to process ƒ Poor reproducibility ƒ High cost Cellular Interactions with Synthetic Degradable Solids Used as Scaffolds? Review older literature looking directly at cells on PLGA, PLA, etc. Molecularly-Designed Surfaces for TE Reconstruction at Scaffold Surfaces Lecture 6 – Biodegradable Polymers in Tissue Engineering 3 of 5