1/17/2016 A comparison of the effectiveness of various Heat Resistance and Thermal Death antiseptics Bacterial endospores are most resistant to heat and 100 require femperatures above boiling fo destroy them. Soap and water 80 TAREE 114 Ihermal Death Iimes of various Endospores 40 Topical antiseptics 12℃ 120C 10填 90 C.NoME 105c 121 13m B.mimberopl l4C Smin t20r℃ 80 t00 120 120min C. TABLE 7.4 Size Moist Heat Methods Steam under pressure-sterilization Contuiner Sire ·Autoclave15 psi at121°Cfor10-40min 18150mm 10 ml Steam must reach surface of item being sterilized yeoak. 95 ml 5 1500ml Item must not be heat or moisture sensitive 0 Mode of action-denaturation of proteins, 58c6oabote 6750ml 70 destruction of membranes and DNA Preferred method of sterilization 61/17/2016 6 A comparison of the effectiveness of various antiseptics Topical antiseptics Heat Resistance and Thermal Death Bacterial endospores are most resistant to heat and require temperatures above boiling to destroy them. Moist Heat Methods • Steam under pressure – sterilization • Autoclave 15 psi at 121 • Autoclave 15 psi at 1210C for 10 40 min 0C for 10-40 min • Steam must reach surface of item being sterilized • Item must not be heat or moisture sensitive • Mode of action – denaturation of proteins of proteins, destruction of membranes and DNA • Preferred method of sterilization