Two kinds of signal The leader of yeast Initiation cytochrome cl contains an N-terminal region Charged leader region for mitochondrial targeting that targets the protein k阿四匣四网回园回 to the mitochondrion followed by a region m四回p回四四圆四 that targets the(cleaved) Cleavage 1 protein to the inner membrane. The leader回四剛回四回國画回回回 is removed by two Continuous uncharged leader region targets inner membrane cleavage events Cleavage 2Two kinds of signal • The leader of yeast cytochrome c1 contains an N-terminal region that targets the protein to the mitochondrion, followed by a region that targets the (cleaved) protein to the inner membrane. The leader is removed by two cleavage events