Challenge How to prepare electron transparent specimen(20-100 nm thick)without introducing artifacts? Providing adequate facilities are available,there is no real barrier to the preparation of good,thin- film specimens from any engineering material, and no excuse whatsoever for wasting time on the examination of a poor specimen in the microscope. -From David Brandon and Wayne D.Kaplan,Microstructural characterization 6 of materialsChallenge Providing adequate facilities are available, there is no real barrier to the preparation of good, thin￾film specimens from any engineering material, and no excuse whatsoever for wasting time on the examination of a poor specimen in the microscope. -From David Brandon and Wayne D. Kaplan, Microstructural characterization of materials 6 How to prepare electron transparent specimen (20-100 nm thick) without introducing artifacts?