II Non-identical and independent binding sites k k k K=k/k K=kA/k KISI K IS] Independent binding: r 1+K[S]1+K[S]II Non-identical and independent binding sites S S S k + k - k + * k - * Independent binding: [S] * 1 K [S] * K 1 K[S] K[S] r + + + = K=k +/k - K *=k + */k - * S k + * k - * k + k -