CLONORCHIS SINENSIA Objectives and requirements 1. To study the morphology and the life history of C sinensis as an example of general feature of trematodes 2. To study the infection route, infection mode, parasitized site and the pathogenesis of C. sinensis infection 3. To study the characteristics of ovum of C. sinensis and the pathogenic diagnosis of C. sinensis infection 4. To learn the basic skill of drawing eggs Observation and experiment 1. Adult worm (1)Observe the preserved specimen(Demonstration) Note the size, color, shape of the organism. Spindle-shaped, transparent (2)Observe the general feature of C. sinensis in stained specimen( Manipulation First with naked eye, then study under microscope, note and identify following A. Adherent organs: oral and ventral suckers, compare their size. B. Digestive system: mouth, pharynx, esophagus and ceca C. Excretory system: excretory bladder and pore D. Reproductive sys tem: hermaphroditism Male: observe the number, size, shape and position of testes Female: observe ovary, seminal receptacle, vitellarium, Mehlis' gland and uterus What is meant by hermaphroditism? 2. Larva and intermediate hosts (Demonstration) (1) See live sporocyst, redia and cercaria(or stained sporocyst to replace live one if (2)See the stained cercaria Note the eye-spots and characteristics of tail lipoidal shape, 138 u mX115 u m, with two suckers(oral sucker an entral sucker) and excretory bladder containing black granules (4)See the first intermediate hosts(Parafossarulus, Alocinma, Bithynia snails), and second intermediate hosts, fresh water fish and crayfish. What are their names? 3. Ovum(Manipulation) (1)Study the ova. Note the shape, color, shell, operculum resting on a rim which takes3 CLONORCHIS SINENSIS Objectives and Requirements 1. To study the morphology and the life history of C. sinensis as an example of general feature of trematodes. 2. To study the infection route, infection mode, parasitized site and the pathogenesis of C. sinensis infection. 3. To study the characteristics of ovum of C. sinensis and the pathogenic diagnosis of C. sinensis infection. 4. To learn the basic skill of drawing eggs. Observation and Experiment 1. Adult worm (1) Observe the preserved specimen (Demonstration). Note the size, color, shape of the organism. Spindle-shaped, transparent. (2) Observe the general feature of C. sinensis in stained specimen (Manipulation). First with naked eye, then study under microscope, note and identify following structures. A. Adherent organs: oral and ventral suckers, compare their size. B. Digestive system: mouth, pharynx, esophagus and ceca. C. Excretory system: excretory bladder and pore. D. Reproductive system: hermaphroditism. Male: observe the number, size, shape and position of testes. Female: observe ovary, seminal receptacle, vitellarium, Mehlis’ gland and uterus. What is meant by hermaphroditism? 2. Larva and intermediate hosts (Demonstration) (1) See live sporocyst, redia and cercaria (or stained sporocyst to replace live one if necessary). (2) See the stained cercaria. Note the eye-spots and characteristics of tail. (3) Live metacercaria. Note its ellipsoidal shape, 138μm×115μm, with two suckers (oral sucker and ventral sucker) and excretory bladder containing black granules. (4) See the first intermediate hosts (Parafossarulus, Alocinma, Bithynia snails), and second intermediate hosts, fresh water fish and crayfish. What are their names? 3. Ovum (Manipulation) (1) Study the ova. Note the shape, color, shell, operculum resting on a rim which takes