Wang et al Anticancer Exopolysaccharide From B breve Iwo1 UDP-galactosephosphotransferase Protein tyrosine phosphatase Membrane spanning protein Glycosyltransferase Thiamine pyrophosphate enzyme Polymerase Acyltransferase Chain length regulator Transcription and Translation Membrane-panning protein Flippase Glycosyltransferase Polymerization Chain length regulator Glucose or Lactose Out 2888 In Lipid carrier Chain length determination 2. Assembling module Glucose or Lactose +GTF 3. Exporting module GDP-mannose UDP-arabinose UDP-glucose +UDP-galactose TDP-rhamnose mannose-6-P arabinose-1-P EMP+Glucose-6-P -Glucose-1-P+ TDP-glucose--TDP-4K-6D-mannosel 1. Precursor synthesis module FIGURE 2 Physical map of the putative EPS cluster of B breve lwo1 and a schematic diagram of EPS biosynthesis in this strain. (A) The genes were categorize ccording to their potential functions, which are indicated with the colored arrows.(B)Hypothetical EPs biosynthesis pathway in strain B breve l01 based on its mology analysis of the EPS cluster. B breve lw01 B breve UCC2003 um NCC2705 B infantis ATCC 15697 B asteroides PRL2011 B/actis DSM 10140 Q qf nv kgdms vgprp ev y I ys riV pgi t gpwq sgr B FIGURE 3 Algnment of an fragment of amino acid sequence of priming- GTF in B breve Mo1 and other Bifidobacterium strains. B at the bottom represented blocks involved in interaction with lipid carrier and c represented blocks involved in sugar-specific recognition. irregular spherical bodies, flakes and nets. EPS was relatively of branches. The use of different methods ough, and was composed of depressions and voids, which may of extraction and purification of EPS could in the different be due to the nature and structure of EPS, and the formation ultrastructure( Figure 5) FrontiersinMicrobiologywww.frontiersin.org May 2019 Volume 10 Article 1044fmicb-10-01044 May 8, 2019 Time: 14:36 # 6 Wang et al. Anticancer Exopolysaccharide From B. breve lw01 FIGURE 2 | Physical map of the putative EPS cluster of B. breve lw01 and a schematic diagram of EPS biosynthesis in this strain. (A) The genes were categorized according to their potential functions, which are indicated with the colored arrows. (B) Hypothetical EPS biosynthesis pathway in strain B. breve lw01 based on its homology analysis of the EPS cluster. FIGURE 3 | Alignment of an fragment of amino acid sequence of priming-GTF in B. breve lw01 and other Bifidobacterium strains. B at the bottom represented blocks involved in interaction with lipid carrier and C represented blocks involved in sugar-specific recognition. irregular spherical bodies, flakes and nets. EPS was relatively rough, and was composed of depressions and voids, which may be due to the nature and structure of EPS, and the formation of branches. The use of different methods in the process of extraction and purification of EPS could cause different ultrastructure (Figure 5). Frontiers in Microbiology | www.frontiersin.org 6 May 2019 | Volume 10 | Article 1044