RESEARCH LETTER a Control 100 nM UK 500 nM UK 0.2 nA L 0.5nA 10 ms 0.2 nA L 10 ms 10 ms 1000nM 5000nW UK-5981 0.5 nAL 0.2nA 10 ms 10 ms b 0.5 0.5 0.0 0.0 10 10 Number of pulses UK-59811(nM） for UK-s981 Datawer uming ofUK-59811 a binding,.ico】 UK-59811 at 0nM (black), nM(red),1uM( 2016 Macmillan Publishers Limited part of Soringer Nature.All tights reservec RESEARCH Letter Extended Data Figure 4 | Biophysical characterization of block by UK-59811. a, Ba2+ currents for state-dependent block by different concentrations of UK-59811. b, State-dependent block of CaVAb by UK-59811 at 0 nM (black), 100 nM (green), 500 nM (red), 1 μM (blue), and 5 μM (brown). For each curve, n=4–5 cells. c, Concentration￾response curve for UK-59811. Data were fit with a Hill equation assuming a 1:1 binding. IC50=194±22 nM, n=4–5. © 2016 Macmillan Publishers Limited, part of Springer Nature. All rights reserved