上游充通大警 SHANGHAI JIAO TONG UNIVERSITY Pasteur and the Downfall of Spontaneous Generation 2nd experiment Method ·Broth in sealed flask 。Heating Results Heated broth separated from the air never spoils Conclusion Spontaneous does not occur,microbial cells can only come from the air/environment. Criticism: Fresh air is needed for spontaneous generation, heating destroyed the power for spontaneous 20 generation in the air sealed in the flask交通大学( 《微生物的世界》20 上海交通大学《微生物的世界》 Downfall of Spontaneous generation (2) Method • Broth in sealed flask • Heating Results • Heated broth separated from the air never spoils Conclusion • Spontaneous does not occur, microbial cells can only come from the air/ environment. Pasteur and the Downfall of Spontaneous Generation 2 nd experiment Criticism: Fresh air is needed for spontaneous generation, heating destroyed the power for spontaneous generation in the air sealed in the flask