Michaelis-Menten equation z If you assume that the formation of ES equals its breakdown, making [ES] constant (steady state), then: Km = k-1 + k2 k 1 v = Vmax • [S] Km + [S] E + S ES E + P k 1 k-1 k2 k1 [E][S] = k-1 [ES] + k2 [ES ]Michaelis-Menten equation z If you assume that the formation of ES equals its breakdown, making [ES] constant (steady state), then: Km = k-1 + k2 k 1 v = Vmax • [S] Km + [S] E + S ES E + P k 1 k-1 k2 k1 [E][S] = k-1 [ES] + k2 [ES ]