Name Section Question 2 Your professor Flow Enirehtac asks you to learn more about the PDGF receptor inside these alien cells. In order to discover the function of various regions in the protein you create cells that no longer express the wild type form of the receptor but various truncated forms You then use an antibody against the PdgF receptor in order to visualize where in these cells the protein is located. You find that the pdgF receptor protein is found in the cytosol of all the cells a)All the truncated forms of the PDGF receptor are missing sequence form one end Which end of the receptor protein is likely to have been deleted? Why would this deletion change the localization of the PDgF receptor b) You look closely at many other cell lines that fail to respond to PDgF i)Type 1 cells have a single gene mutation and fail to respond to all growth factors Antibody staining of these cells shows that many different membrane proteins are now found free in the cytoplasm. Which component(s)of the protein secretion pathway might be missing in type 1 cells ii)What other kinds of proteins might be affected in Type 1 cells? i)Type 2 cells do not respond to PDGF because PDGF receptor proteins are secreted out of the cell. what kind of mutation would lead to this result? 7012fall2003Name:___________________________________ Section:_____ 7.012 fall 2003 3 Question 2 Your professor Flow Enirehtac asks you to learn more about the PDGF receptor inside these alien cells. In order to discover the function of various regions in the protein you create cells that no longer express the wild type form of the receptor but various truncated forms. You then use an antibody against the PDGF receptor in order to visualize where in these cells the protein is located. You find that the PDGF receptor protein is found in the cytosol of all the cells. a) All the truncated forms of the PDGF receptor are missing sequence form one end. • Which end of the receptor protein is likely to have been deleted? • Why would this deletion change the localization of the PDGF receptor? b) You look closely at many other cell lines that fail to respond to PDGF. i) Type 1 cells have a single gene mutation and fail to respond to all growth factors. Antibody staining of these cells shows that many different membrane proteins are now found free in the cytoplasm. Which component(s) of the protein secretion pathway might be missing in Type 1 cells. ii) What other kinds of proteins might be affected in Type 1 cells? iii) Type 2 cells do not respond to PDGF because PDGF receptor proteins are secreted out of the cell. What kind of mutation would lead to this result?