FAME Analysis Procedure IDENTIFY ORGANISM ↑ Compare pattern of peaks with patterns in database Bacterial culture Extract fatty acids Peaks from various fatty acid methyl esters Derivatize to form methyl esters Gas chromatography (b) 2012 Pearson Education,inc Each peak from the gas chromatograph is due to one particular fatty acid methyl ester and the peak height is proportional to the amount.FAME Analysis Procedure Each peak from the gas chromatograph is due to one particular fatty acid methyl ester and the peak height is proportional to the amount