Human genome DNA Extraction Venous blood(5m)→WBC→ lysing buffer (sds) and protease K-y centrifugation. collect supernatant -> phenol -> centrifugation, collect supernant→ RNase a and t1→ phenol > centrifugation, collect supernatant otassium acetate and etoH (absolute)->genomic DNA appears (like cotton fiber)Human genome DNA Extraction • Venous blood(5 ml) → WBC → lysing buffer (SDS) and protease K → centrifugation, collect supernatant → phenol → centrifugation, collect supernant → RNase A and T1 → phenol → centrifugation, collect supernatant → potassium acetate and EtOH (absolute) → genomic DNA appears (like cotton fiber)