生物大分子波谱学原理 吴季辉 TOCSY D 应用 TOCSY可提供整个自旋体系的连接，而且信号强， 对于谱峰证认很有用。 8 Y回 Y回 6 p Y马 B"⑤ B"@ BO …B③ @ 回 8 @ @ 8 分 o O龙@eo K48 K29 K48 K29 K48 K29 8.0 7.9 8. 7.9 8.0 7.9 F2(ppm) FIGURE 6.45 Sections of H2O TOCSY spectra acquired with mixing times of 48 (left),83(center),and 102 ms (right).The cross-peaks observed to the amide protons of Lys29 and Lys48 are assigned at the different mixing times.Cross- peaks to the spin-system termini are observed only at the longer mixing times.TOCSY 生物大分子波谱学原理 吴季辉 D 应用 TOCSY可提供整个自旋体系的连接，而且信号强， 对于谱峰证认很有用