Cell survival Cell survival No transport across Drug hydrolyzed Cell wall outer membrane pre cursors Penicillin-binding Environment >Outer Periplasmic proteins(PBPs) space membrane ↓ catalyze synthesis e.g. transpeptidases β- actam antibiotic Binds carboxy peptidases PBPs endopeptidases peptidoglycan Cell death B-lactam antibiotic Falls to blind Mutant PBPsCell survival GRAM-NEGATIVE BACTERIA6