Name TA b)Predict whether the following F. fabricatus strains, that are merodiploid for the ridiculose operon, will grow on minimal media with or without ridiculose as the only carbon source Fill in the chart with yEs if the merodiploid will GROW or No if the merodiploid will NOT GROW 7 Pts (5 points for the first column, 2 pts for entire last column) eroalbIol Ridiculose Ridiculose WT/WT yES NO M1/M2 (deletion of Puvx NO control region that can't bind Ridw) M2/M3 ( control region that can't bind Ridw) yES NO (Ridw protein that can't bind ridiculose) M3/M1337 (Ridw protein that can't bind ridiculose) yES NO (RidW protein that always binds control region) M4/M1 sense mutation early in rid) (deletion of Puvx M1337/M4 (RidW protein that always binds control region) (nonsense mutation early in ridX)Name:___________________________________ TA:_______________________ 3 b) Predict whether the following F. fabricatus strains, that are merodiploid for the ridiculose operon, will grow on minimal media with or without ridiculose as the only carbon source. Fill in the chart with YES if the merodiploid will GROW or NO if the merodiploid will NOT GROW 7 pts (5 points for the first column, 2 pts for entire last column) Merodiploid +Ridiculose - Ridiculose _______________________________________________________________ WT / WT YES NO _______________________________________________________________________ M1 / M2 (deletion of PUVX) NO NO (control region that can’t bind RidW) _______________________________________________________________________ M2/ M3 (control region that can’t bind RidW) YES NO (RidW protein that can’t bind ridiculose) _______________________________________________________________________ M3 / M1337 (RidW protein that can’t bind ridiculose) YES NO (RidW protein that always binds control region) _______________________________________________________________________ M4 / M 1 (nonsense mutation early in ridX) NO NO (deletion of PUVX) _______________________________________________________________________ M1337 / M4 (RidW protein that always binds control region) YES NO (nonsense mutation early in ridX) _______________________________________________________________