Syntheic gene fragment, coding Bacterial cell i stage 3 Insert the human insulin gene into E. coli and culture the recombinant Ecoli to produce insulin (trade name= Humulin) Mitosis. Yeast is also used to produce insulin (trade name 0000 Novolin⑧)(1987) Recombinant dna technology has also made it possible to manufacture slightly-modified forms of human insulin that work faster (Humalog& and novolog )or slower (lantus) than regular human insulin◼ Stage 3 Insert the human insulin gene into E. coli and culture the recombinant E.coli to produce insulin (trade name = Humulin®). Yeast is also used to produce i n s u l i n ( t r a d e n a m e = N o v o l i n ® ) ( 1987 ) . Recombinant DNA technology has also made it possible to manufacture slightly-modified forms of human insulin that work faster (Humalog® and NovoLog®) or slower (Lantus®) than regular human insulin