☆ Active transport limited proteolysis radioactive radioactive radioactive colloidal gold particle nucleoplasmin coated with tails MICROINJECTION INTO CYTOSOL OF XENOPUS OOCYTE 0)(a(8 nucleus. INCUBATION DETECTION BY AUTORADIOGRAPHY DETECTION BY EM UPTAKE INTO UPTAKE INTO NUCLEUS UPTAKE NOCLEUS UPTAKE INTO NUCLEUS > Transport of large proteins into nucleus needs nuclear localization signal (NLs)❖ Active transport ➢Transport of large proteins into nucleus needs nuclear localization signal (NLS)