Axial resolution- Depth of Field Depth of focus (f mm) Depth of Field ranges(F um) Low Depth of focus Image plane High object specimen Plane NA fF 0.10.1315.5Deph 04385.8 of Field 9580.00.19 Object plane The distance above and below The axial range through which geometric image plane within an object can be focused without which the image is in focus any appreciable change in image MI NAIf F sharpness F is determined by Na http://www.matter.org.uk/tem/depth_of_field.htm http://www.youtube.com/watch?v=fvc2wluqeugatw3:40Depth of focus (f mm) The distance above and below geometric image plane within which the image is in focus The axial range through which an object can be focused without any appreciable change in image sharpness (F m) M NA f F M NA f F Axial resolution – Depth of Field Depth of Field Ranges (F m) F is determined by NA. NA f F 0.1 0.13 15.5 0.4 3.8 5.8 .95 80.0 0.19 http://www.matter.org.uk/tem/depth_of_field.htm http://www.youtube.com/watch?v=FvC2WLUqEug at~3:40