2)actos 3)allolactose 4) CRP 11. Which of the following RNA species is involved in degradation of the mRNA containing complementary sequence 1)miRNA 3)tRNA 4)5S RNA 5)U3 snRNA 12. The genome sequencing projects are confirming the theory that genome size is directly proportional to the number of genes contained within that genome. In other words, a genome that is 10 times as big will contains approximately 10 times as many protein cod ing genes D)True 2) False 13. HeLa cells, derived from a human cervical carcinoma, are able to propagate indefinitely in culture and are therefore known as a(n) 1)tissue culture 2)tu 3) transgenic cell line 4) immortalized cell line 14. E. coli cells are smaller than yeast cell 15. Which of the following domains is not a DNA binding domain 2)Helix-turn helix domains 3)Zinc finger domains 4)Basic domains 16. The aminoacyl-tRNA synthetases distinguish between about 40 different shaped trna molecules in the cells 2 False PART III: SHORT QUESTIONS(8 points each) 1. How do bacterial replication start and accomplished. Remember to include the proteins/enzymes and important DNa sequence involved in this process Initiation (3 points): O replication of the bacterial chromosome is tightly coupled to the growth cycle 2 The E coli origin is within the genetic locus oriC that contains four 9 bp binding sites for the initiator protein dnaA Synthesis of dnaA is coupled to growth rate so that the replication of the bacterial chromosome is coupled to the growth cycle 4 Once the cellular level of DnaA reaches a critical level, DnaA protein forms a complex of 30-40 molecules at the oriC DNA, which facilitates melting of three 13 bp AT-rich repeat sequences to allow binding of DnaB protein2) lactose 3) allolactose 4) CRP 11. Which of the following RNA species is involved in degradation of the mRNA containing complementary sequence 1) miRNA 2) siRNA 3) tRNA 4) 5S RNA 5) U3 snRNA 12. The genome sequencing projects are confirming the theory that genome size is directly proportional to the number of genes contained within that genome. In other words, a genome that is 10 times as big will contains approximately 10 times as many protein coding genes. 1) True 2) False 13. HeLa cells, derived from a human cervical carcinoma, are able to propagate indefinitely in culture and are therefore known as a(n): 1) tissue culture. 2) tumor. 3) transgenic cell line. 4) immortalized cell line. 14. E. coli cells are smaller than yeast cells. 1) True 2) False 15. Which of the following domains is not a DNA binding domain 1) Proline-rich domains 2) Helix-turn helix domains 3) Zinc finger domains 4) Basic domains 16. The aminoacyl-tRNA synthetases distinguish between about 40 different shaped tRNA molecules in the cells. 1) True 2) False PART III: SHORT QUESTIONS (8 points each) 1. How do bacterial replication start and accomplished. Remember to include the proteins/enzymes and important DNA sequence involved in this process. Initiation (3 points): ① replication of the bacterial chromosome is tightly coupled to the growth cycle. ② The E. coli origin is within the genetic locus oriC that contains four 9 bp binding sites for the initiator protein DnaA. ③ Synthesis of DnaA is coupled to growth rate so that the replication of the bacterial chromosome is coupled to the growth cycle. ④ Once the cellular level of DnaA reaches a critical level, DnaA protein forms a complex of 30-40 molecules at the oriC DNA, which facilitates melting of three 13 bp AT-rich repeat sequences to allow binding of DnaB protein