Temperature z Freezing – Activity depends on the enzyme (0 to –10C) – Below –10C almost always decrease activity z Factors involved in inconsistent behavior – Composition of medium – Rate and extent of freezing – Concentration effects – Viscosity – Changes in phase (crystallization of water, solidification of triacylglycerides)Temperature z Freezing – Activity depends on the enzyme (0 to –10C) – Below –10C almost always decrease activity z Factors involved in inconsistent behavior – Composition of medium – Rate and extent of freezing – Concentration effects – Viscosity – Changes in phase (crystallization of water, solidification of triacylglycerides)