E The intron is removed in a Form called a lariat(套马索)as o the Flanking Exons are joined Two successive transesterification: 2 Step 1: The OH of the conserved A o at the branch site attacks the phosphoryl group of the conserved G in the 5, splice site. g As a result, the 57 exon is released and the 5-end of the 2. intron forms a three-way 日 junction structureThe intron is removed in a Form Called a Lariat (套马索) as the Flanking Exons are joined Two successive transesterification: Step 1: The OH of the conserved A at the branch site attacks the phosphoryl group of the conserved G in the 5’ splice site. As a result, the 5’ exon is released and the 5’-end of the intron forms a three-way junction structure. The chemistry of RNA splicing