CDNA ArraysⅣ- Scanning Laser excitation of hybridized targets-emission spectra measured using a scanning confocal laser microscope 2. Monochrome images(from scanner) are imported into software in which images are pseudo colored and merged 3. Data analyzed as normalized ratio ( Cy3/Cy5)-gene expression increase or decrease relative to reference sample Please see Duggan, DJ, M Bittner, Y Chen, P Meltzer, and JM Trent. " Expression Profiling using CDNA Microarrays. "Nat Genet. 21, no. 1 Suppl (January 1999 ) 10-41. Laser excitation of hybridized targets - emission spectra measured using a scanning confocal laser microscope 2. Monochrome images (from scanner) are imported into software in which images are pseudo￾colored and merged 3. Data analyzed as normalized ratio (Cy3/Cy5) - gene expression increase or decrease relative to reference sample cDNA Arrays IV - Scanning Duggan, DJ, M Bittner, Y Chen, P Meltzer, and JM Trent. "Expression Profiling using cDNA Microarrays." Nat Genet. 21, no. 1 Suppl (January 1999): 10-4. Please See