uestion The following double-stranded dNA contains sequence of a eukaryotic gene b123 5-ATGGCCTTCACACAGGAAACAGCTATGGCCATGAGCACGC 3'-TACCGGAAGTGTGTCCTTTGTCGATACCGGTACTCGTGCG CAGTCTCGGCAT TATCCTATTAAAGGGAACTGAGGTGA-3 GTCAGAGCCGTAATAGGATAATTTCCCTTGACTCCACT-5 a)Transcription begins at the underlined A/T at base pair 17(b)and proceeds to the right What are the first 12 nucleotides of the resulting mRNA? Indicate the 5 and 3 ends of the mrNA b)The first 7 amino acids of the protein encoded by this gene are NH3+-met-ala-met-ser-thr-pro-his-tyr.COO- i)underline the nucleotides which correspond to the 5 untranslated region of the primary RNA transcript made from this gene ii)draw a box around the intron region in this gene c) Consider each of the following three mutations independently i)How would the resulting protein change if the underlined G/C base pair at position 22 (1) was deleted from the DNA sequence? Briefly explain was changed to a C/g base pair? Briefly expla underlined G/C base pair at position 27(2) ii)How would the resulting protein change if the iii)How would esulting protein change if the underlined A/T base pair at position 31 (3)was deleted d from the DNA sequence? Briefly explain Spring 2004 Final exam practiceSpring 2004 Final Exam Practice 4 Question 2 The following double-stranded DNA contains sequence of a eukaryotic gene: b 1 2 3 5'-ATGGCCTTCACACAGGAAACAGCTATGGCCATGAGCACGC 1 ---------+---------+---------+---------+ 40 3'-TACCGGAAGTGTGTCCTTTGTCGATACCGGTACTCGTGCG ii CAGTCTCGGCATTATCCTATTAAAGGGAACTGAGGTGA-3' 41 ---------+---------+---------+---------+ 80 GTCAGAGCCGTAATAGGATAATTTCCCTTGACTCCACT-5' i a) Transcription begins at the underlined A/T at base pair 17 (b) and proceeds to the right. What are the first 12 nucleotides of the resulting mRNA? Indicate the 5' and 3' ends of the mRNA. b) The first 7 amino acids of the protein encoded by this gene are: NH3+ -met-ala-met-ser-thr-pro-his-tyr....COO￾i) underline the nucleotides which correspond to the 5' untranslated region of the primary RNA transcript made from this gene. ii) draw a box around the intron region in this gene. c) Consider each of the following three mutations independently. i) How would the resulting protein change if the underlined G/C base pair at position 22 (1) was deleted from the DNA sequence? Briefly explain. ii) How would the resulting protein change if the underlined G/C base pair at position 27 (2) was changed to a C/G base pair? Briefly explain. iii) How would the resulting protein change if the underlined A/T base pair at position 31 (3) was deleted from the DNA sequence? Briefly explain