Name Section Question 1, continued c)Treating a population of normal stem cells with a light dose of X-rays can"mark"each cel with a distinctive tag. This light dose will not kill the stem cells but instead will create random breaks and rearrangements of chromosomes that can be seen under the light microscope. As a consequence, each of the cells in such a lightly irradiated population w ill have a distinctive unique chromosomal abnormality that serves to distinguish it from its sister cells in the population. You create a population of"marked"stem cells to be used as donor cells in the rescue of lethally irradiated mice i)When you examine the blood cells of the first rescued mouse you find that all Basophils, Neutrophils, and Eosinophils have the same chromosomal marker. In the diagram, circle the type of donor cell where chromosomal rearrangement first occurred? ii)When you examine the blood cells of the second rescued mouse you find that all Basophils, Neutrophils, Eosinophils, and monocytes have the same chromosomal marker and all megakaryocytes have a different marker. Ho stem cells from the donor population had a rearrangment ow many different kinds of One iii) You examine the blood cells of the last rescued mouse. You realize that this mouse hows the existence of a pluripotent bone marrow stem cell. What did find in this mouse? The macrophages, Basophils, Neutrophils, Eosinophils, Monocytes, Red Blood Cells, and Megakaryocytes all have the same marker 7012Fall2003Name:___________________________________ Section:_____ 7.012 Fall 2003 2 Question 1, continued c) Treating a population of normal stem cells with a light dose of X-rays can “mark” each cell with a distinctive tag. This light dose will not kill the stem cells but instead will create random breaks and rearrangements of chromosomes that can be seen under the light microscope. As a consequence, each of the cells in such a lightly irradiated population will have a distinctive, unique chromosomal abnormality that serves to distinguish it from its sister cells in the population. You create a population of “marked” stem cells to be used as donor cells in the rescue of lethally irradiated mice. i) When you examine the blood cells of the first rescued mouse you find that all Basophils, Neutrophils, and Eosinophils have the same chromosomal marker. In the diagram, circle the type of donor cell where chromosomal rearrangement first occurred? ii) When you examine the blood cells of the second rescued mouse you find that all Basophils, Neutrophils, Eosinophils, and Monocytes have the same chromosomal marker and all megakaryocytes have a different marker. How many different kinds of stem cells from the donor population had a rearrangment? One iii) You examine the blood cells of the last rescued mouse. You realize that this mouse shows the existence of a pluripotent bone marrow stem cell. What did find in this mouse? The Macrophages, Basophils, Neutrophils, Eosinophils, Monocytes, Red Blood Cells, and Megakaryocytes all have the same marker