Laboratory process development Shake Flask experiments Optimization of conditions for cell growth and product formation using shake flask experiments 1. pH 2. Temperature 3. Dissolved oxygen(DO) 4. Substrate choice 5. Maximal and optimal substrate concentration 6. OthersLaboratory process development Shake Flask Experiments Optimization of conditions for cell growth and product formation using shake flask experiments: 1. pH 2. Temperature 3. Dissolved oxygen (DO) 4. Substrate choice 5. Maximal and optimal substrate concentration 6. Others