Restriction Enzyme Specificity e.g Eco RI: GAATTC Bam HI GGA“6- cutters Frequency: 4b= average of 1 cut site per 4,096 bp e.g. Sau 3A GATC “4- cutter Frequency: 44=average of 1 cut site per 256 bp e.g. Not I GCGGCCGO“8- cutter Frequency: 48=average of 1 cut site per 65, 536Restriction Enzyme Specificity • e.g. Eco RI: GAATTC Bam HI GGATCC “6-cutters” • Frequency: 46 = average of 1 cut site per 4,096 bp • e.g. Sau 3A GATC “4-cutter” • Frequency: 44 = average of 1 cut site per 256 bp • e.g. Not I GCGGCCGC “8-cutter” • Frequency: 48 = average of 1 cut site per 65,536