Systematic expansion of the regulatory network In order to improve the predictive ability of the in silico model, ChIP-chip and TF-binding motif data were used to systematically expand the regulatory network part of the model First, the gene expression comparison presented above was used to identify potential novel candidate target genes for each of the 10 transcription factors(498TF target pairs total) Next, we traced paths through an expanded regulatory network that consisted of the mh805/775 network and a provisional regulatory network that can be established based on combining ChlP-chip data and TF-binding motif dataSystematic expansion of the regulatory network • In order to improve the predictive ability of the in silico model, ChIP-chip and TF-binding motif data were used to systematically expand the regulatory network part of the model. • First, the gene expression comparison presented above was used to identify potential novel candidate target genes for each of the 10 transcription factors (498 TF￾target pairs total). • Next, we traced paths through an expanded regulatory network that consisted of the iMH805/775 network and a provisional regulatory network that can be established based on combining ChIP-chip data and TF-binding motif data