Enzyme-Linked Immunosorbent Assay(ELISA) and transfer 0.1ml Virus preparation Virus concentration Substract Substract Substract Substract Secondary antibody conjugate Primary antibody conjugate g Captue antibody Direct In Direct Sandwich CompetitiveEnzyme-Linked Immunosorbnent Assay (ELISA) Virus preparation Virus concentration Substract Substract Substract Substract Secondary antibody conjugate Ag Primary antibody conjugate Captue antibody Inhibitor Ag Direct InDirect Sandwich Competitive