DNA Sequencing Two methods of DNA sequencing are available The Maxam-Gilbert method Uses chemical techniques Sanger dideoxy method Uses enzymatic reactions The more commonly used of the two Method responsible for sequencing the entire human genome of 3.0 billion base pairs In commercial sequencing instruments,the dideoxy method begins with a mixture of the following: The restriction fragment to be sequencedTwo methods of DNA sequencing are available ▪ The Maxam-Gilbert method ▪ Uses chemical techniques ▪ Sanger dideoxy method ▪ Uses enzymatic reactions ▪ The more commonly used of the two ▪ Method responsible for sequencing the entire human genome of 3.0 billion base pairs ▪ In commercial sequencing instruments, the dideoxy method begins with a mixture of the following: ▪ The restriction fragment to be sequenced DNA Sequencing