天目山穹宇萤的活动与自然发光规律及其对单色光刺激的反应 天目山穹宇萤的活动与自然发光规律及其对单色光刺 激的反应 杨立峰张晴柔张郑宇”赵倩茹“赵祥 (复旦大学生命科学学院,上海200433) 摘要:以往的实验观察表明,萤火虫的夜晚活动及其发光有一定的变化规律,在日落后一小 时达到发光和活动高峰:部分种类的萤火虫对外界单色光的刺激能做出闪光反应。为了进一 步验证与研究上述的观察结果,我们在天目山对萤科昆虫穹宇萤进行了实地观察与研究。我 们采用的方法是从日落开始,每隔15分钟对观测区域内的自然发光的萤火虫数量以及萤火 虫总数进行计数,每次计数1分钟,连续记录三个1分钟;待发光规律稳定后,再变成每隔 30分钟进行计数。另外,在日落后1小时内,我们还利用单色光绿光、红光、黄光和蓝光 以及白光对萤火虫进行光刺激,观察萤火虫的反应。实验结果表明,穹宇萤在日落后1小时 内活动最强,发光最频繁,之后活动与发光频率逐渐下降:绿光、红光和黄光刺激可以引起 穹宇萤的闪光回应,蓝光和白光无法引起穹宇萤的闪光回应,先用黄光刺激在用白光刺激会 引起穹宇萤闪光的停止。城市光污染会影响萤火虫的正常繁殖活动,引起萤火虫的绝灭。 关键词:方差分析,自然发光数,萤火虫总数,光污染 The activities and natural flashing and the response to monochromatic light stimulus of Pygoluciola qingyu in Tianmu Mountain Lifeng Yang, Qingrou Zhang2, Zhengyu Zhang, Qianru Zhao, Xiang Zhao School of life Sciences, Fudan University, Shanghai, P.R. China 200433 Abstract: From the previous research and observation about fireflies, their activities and natural Their activities and flashing come to the peak at one hour after sunset an decrease as the time gets late. Some species of fireflies are responsive to monochromatic light stimulus, and display synchronous flashing. In order to make further test and studies on these results, we studied and observed the Pygoluciola qingyu in Tianmu Mountain. The method that we used is to count the fireflies which were flashing and the total number of fireflies at that time in the research region at continuously. When the fluctuation became less obvious, we counted at intervals of 30 minutes Besides, we also observed the response of Pygoluciola gingyu to the green light, red light, yellow light, blue light and white light stimulus. Our experimental results present that the Pygoluciola qingyu has its highest frequency of activities and natural flashing in one hour after sunset and after that, the frequency of activities and natural flashing come down. The stimulus of green light, red light and yellow light can lead to the flashing response of Pygoluciola qingyu. If the Pygoluciola qingyu accepts the yellow light 3共同第一作者 Author for correspondence. E-mail:0930070001 Fudan. edu.cn天目山穹宇萤的活动与自然发光规律及其对单色光刺激的反应 天目山穹宇萤的活动与自然发光规律及其对单色光刺 激的反应 杨立峰 1* 张晴柔 2* 张郑宇 3* 赵倩茹 4* 赵祥 5*3 （复旦大学生命科学学院，上海 200433） 摘要：以往的实验观察表明，萤火虫的夜晚活动及其发光有一定的变化规律，在日落后一小 时达到发光和活动高峰；部分种类的萤火虫对外界单色光的刺激能做出闪光反应。为了进一 步验证与研究上述的观察结果，我们在天目山对萤科昆虫穹宇萤进行了实地观察与研究。我 们采用的方法是从日落开始，每隔 15 分钟对观测区域内的自然发光的萤火虫数量以及萤火 虫总数进行计数，每次计数 1 分钟，连续记录三个 1 分钟；待发光规律稳定后，再变成每隔 30 分钟进行计数。另外，在日落后 1 小时内，我们还利用单色光绿光、红光、黄光和蓝光 以及白光对萤火虫进行光刺激，观察萤火虫的反应。实验结果表明，穹宇萤在日落后 1 小时 内活动最强，发光最频繁，之后活动与发光频率逐渐下降；绿光、红光和黄光刺激可以引起 穹宇萤的闪光回应，蓝光和白光无法引起穹宇萤的闪光回应，先用黄光刺激在用白光刺激会 引起穹宇萤闪光的停止。城市光污染会影响萤火虫的正常繁殖活动，引起萤火虫的绝灭。 关键词：方差分析，自然发光数，萤火虫总数，光污染 The activities and natural flashing and the response to monochromatic light stimulus of Pygoluciola qingyu in Tianmu Mountain Lifeng Yang1 , Qingrou Zhang2 , Zhengyu Zhang3 , Qianru Zhao4 , Xiang Zhao5 School of Life Sciences, Fudan University, Shanghai, P.R.China 200433 Abstract: From the previous research and observation about fireflies, their activities and natural flashing at night are regular. Their activities and flashing come to the peak at one hour after sunset and decrease as the time gets late. Some species of fireflies are responsive to monochromatic light stimulus, and display synchronous flashing. In order to make further test and studies on these results, we studied and observed the Pygoluciola qingyu in Tianmu Mountain. The method that we used is to count the fireflies which were flashing and the total number of fireflies at that time in the research region at intervals of 15 minutes; the length of every counting time is 1 minute and we counted three times continuously. When the fluctuation became less obvious, we counted at intervals of 30 minutes. Besides, we also observed the response of Pygoluciola qingyu to the green light, red light, yellow light, blue light and white light stimulus. Our experimental results present that the Pygoluciola qingyu has its highest frequency of activities and natural flashing in one hour after sunset and after that, the frequency of activities and natural flashing come down. The stimulus of green light, red light and yellow light can lead to the flashing response of Pygoluciola qingyu. If the Pygoluciola qingyu accepts the yellow light 3共同第一作者 Author for correspondence. E-mail: 09300700031@fudan.edu.cn 123