Alkaline lysis minipreps of Plasmid dna Principle Alkaline Lysis procedure is to take advantage of the alkaline denaturation of plasmid and chromosomal dna nd of the selective renaturation of plasmid dNa following neutralization of the solution17 Principle: Alkaline Lysis procedure is to take advantage of the alkaline denaturation of plasmid and chromosomal DNA and of the selective renaturation of plasmid DNA following neutralization of the solution. Alkaline Lysis Minipreps of Plasmid DNA