Flow Cytometry Uses A mixture of cells is treated to Differences in electrical label cells that have certain 0 antigens with fluorescent- antibody markers. conductivity between species 00 Fluorescently Cell mixture leaves nozzle in labeled cells droplets. 。Fluorescence of some Laser beam strikes each droplet. species Laser beam Laser Detector of scattered light Cells selectively stained Electrode Fluorescence detector identifies with antibody fluorescent cells by fluorescent Fluorescence light emitted by cell detector fluorescent dye Electrically Electrode gives positive charge to identified cells. As cells drop between electrically charged plates,the cells with a positive charge move closer to the negative plate. ⑦The separated cells fall into different Collection collection tubes. tubes Figure 18.11 • Differences in electrical conductivity between species • Fluorescence of some species • Cells selectively stained with antibody + fluorescent dye Flow Cytometry Uses Figure 18.11