CDNA( complementor DNA):以mRNA为 模板,在反转录酶作用下合成的DNA。 AA-A-A 3 mRNA 3'T-T-T-T5 Oligo(dT) primer (1)Incubate with reverse transcriptase to synthesize mRNA (2 When cDNA strand is completed, hydrolyze RNA strand TTTTTIITT cDNA 3 Incubate with DNA polymerase to synthesize second DNA ran cuts Loose (IlllllIIIIIIIIIIIIIIi dNA e-stranc 4)Incubate with terminal transferase to add single-stranded tails Ill -C-C-C Double-stranded cDNA ready for insertion into appropriate cloning vector CAdd son Wasay Longman. inccDNA（complementory DNA）：以mRNA为 模板，在反转录酶作用下合成的DNA