34监测蛋白质 Monitoring proteins MB SSB Application of MBs in enzymatic Binding、Q 号埠 studies a) Real-time monitoring of SSB-DNA binding: The MB binds to the ssb protein Cleavage whereby its structure is MB Enzyme disrupted and its fluorescence is Cutting restored b)detection of the enzymatic o digestion of DNA: The enzyme cleaves the mb and destroys the hairpin structure to restore MB LDH fluorescence Binding c)Detection of LDH-DNA x interactions: LDH binds the MB oo and disturbs its structure whereby fluorescence is enhanced3.4 监测蛋白质(Monitoring proteins) Application of MBs in enzymatic studies. a) Real-time monitoring of SSB–DNA binding: The MB binds to the SSB protein whereby its structure is disrupted and its fluorescence is restored. b) Detection of the enzymatic digestion of DNA: The enzyme cleaves the MB and destroys the hairpin structure to restore fluorescence. c) Detection of LDH–DNA interactions: LDH binds the MB and disturbs its structure, whereby fluorescence is enhanced