Contents N0ti沁.4 Experimental Report 4,4 PART I:experiments PROTEINS 1.Protein concentration determination.5 Protein Determination by UV Absorption The Lowry Method for Protein Quantitation The Bicinchoninic Acid(BCA)Assay for Protein Quantitation 2.Purification of Y-globulin from human serum.14 3.Cellulose acetate membrane electrophoresis of serum protein.16 ENZYME 4.Extraction and Purification of Alkline 5.Specific Activity of Alkline Phosphatase. .23 6.Enzyme Kinetics:Substrate Concentration Affecting Enzyme Activity -Michaelis Constant Assay of Alkline Phosohatase. .28 NUTRITION METABOLISM 7.The measurement of the blood sugar and effects of adrenalin on blood sugar concentration.32 8.Determinations of Total Serum Triglyceride 35 9.Identification of the transamination by paper chromatography.37 10.Determination of arginase activity of the liver .40 MOLECULAR BIOCHEMISTRY 11.Isolation and Purification of Nucleic Acid44 12.Agarose Gel Electrophoresis of DNA 46 PART II:Principles of Biochemistry experiments Chap.1,Basic Theories of biochemistry experiment.49 Chap.2.Spectrophotometry .58 Chap.3.Electrophoresis .60