等电聚胶电泳 (isoelectric focusing gel IEF) An ampholyte solution is pH9 incorporated into a gel. Hd urseaned pH3 8 A stable pH Protein After staining, gradient is solution is proteins are established in added and shown to be the gel after electric field distributed along application of is reapplied. pH gradient an electric according to field. their pl values. 等电聚胶电泳 (isoelectric focusing gel IEF)