The 16S rRNA or Organismic Isolate DNA 18S rRNA Techniqe ue 16 S RNA gene種 Heat to separate strands: add specific primers Primer extension with DNA polymerase Repeat above steps for many PCR cycles to yield multiple copies of 1 6S ribosomal RNA gene Run agarose gel and check for correct sized PCR product Purify PCR product Sequence by sanger methodThe 16S rRNA or 18S rRNA Technique