12.2 Origins can be mapped by autoradiography and electrophoresis Simple Y Double Y Bubble Asymmetric bubble Figure 12.6 The position of the origin and the Fragment size doubles during number of replicating replication <><< forks determine the shape of a replicating restriction fragment, which can be followed by its Second dimension electrophoretic path(solid exaggerates contribution line). The dashed line of 3D shape 2 kb 2kb、12kt- shows the path for a linear 1 kb 1 kb 1 kb 1 kb DNA First dimension separates by 消当Figure 12.6 The position of the origin and the number of replicating forks determine the shape of a replicating restriction fragment, which can be followed by its electrophoretic path (solid line). The dashed line shows the path for a linear DNA. 12.2 Origins can be mapped by autoradiography and electrophoresis