h 上游充通大睾 inoculum of test bacteria growth medium+ antibiotic antibiotic 16 4 2 0.5 0.25 0.12mg/1 concentration overnight incubation read MIC MIC of antibiotic =1 mg/l subculture to drug-free 16 8 2 0.5· 0.25 0.12mg/ agar overnight incubation MIC of read MBC antibiotic 2 mg/l Fig.30.35 More precise measures of the amount of antibiotic the standard method as outlined in this illustration,the MIC result is required to inhibit and kill a bacterial population can be estimated available after 24 hours and the MBC result after 48 hours.A by establishing the minimum inihibitory concentration(MIC)and number of variables such as the inoculum size,the growth medium minimum bactericidal concentration(MBC)of the antibiotic.Using and the interpretation of the results affect the results of MIC tests.Shanghai Jiao Tong University